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Image Search Results
Journal:
Article Title: Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages
doi: 10.1128/JVI.77.18.10125-10130.2003
Figure Lengend Snippet: MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor TNF-α-induced NF-κB nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.
Article Snippet: BM macrophages were mock or MCMV infected for 18 h and then stimulated with TNF-α (10 ng/ml) for 30 min. Macrophages were then fixed, washed, and stained for NF-κB by using the
Techniques: Translocation Assay, Quantitative RT-PCR, Immunofluorescence, Infection